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  Morphology

Morphology:

Since of the lack of cell wall, morphology is not specific. Growth in fluid media gives mount to numerous different forms such as rings, filaments and granules bacillary and spiral bodies. Growth on solid medium exhibits plastic protoplasmic masses of vague shape and are easily distorted.

Culture:

Mycoplasma grows in heart infusion peptone broth with 2 percent agar, pH 7.8 to which 30 percent human ascitic fluid or animal serum is added. Incubation at 37°C for 48 to 96 hrs reveals no turbidity in fluid however centrifuged sediments illustrate pleomorphism in Giemsa stain.

Growth Characteristics:

Very small in size. They grow on complex however cell free media. They pass via filters and are comparable with Chlamydia or big viruses. Many Mycoplasmas employ glucose as a source of energy and Urea plasmas need urea [10%]. They grow on cell free media that have lipoprotein and sterol.

Classification:

They belong to the group Mollicutes (i.e., soft skin). They lack cell wall and cell wall precursors. Mollicutes are categorized into 2 orders:

(1) Mycoplasmatales and
(2) Acholeplasmatales.

Mycoplasmatales are further divided into two groups:

(a) Mycoplasmataceae and
(b) Spiroplasmataceae.

Mycoplasmataceae contain two genera:

(a) Mycoplasma and
(b) Ureaplasma.

 

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