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  Elementary and reticulate body difference

Differences between elementary and reticulate body:

Elementary body                             Reticulate body (Initial body)

Size about 0.3µm                            0.5 µm
Rigid cell wall                                  Fragile cell wall
Relatively resistant to sonication       Sensitive to sonication
Resistant to trypsin                          Lysed by trypsin
Subunit in cell envelope                   No sub unit in envelope
RNA: DNA content is 1:1                  RNA: DNA is 3:1
Toxic for mice                                 Not toxic for mice
Infectious                                       Not infectious
Adapted for extracellular survival     Adapted for intracellular growth

Structure and chemical composition:

Test of highly purified materials reveals:

Outer cell wall:

It looks like the cell wall of Gram negative bacteria, consists of relatively high lipid content.

It is firm and does not have typical bacterial peptido glycan. The tetra peptide related matrix is seen. Penicillin binding proteins take place in chlamydiae. Cell wall formation is inhibited by cycloserine and penicillins and substances which inhibit transpeptidation. Lysozyme has no effect on chlamydial cell wall and N-acetyl muramic acid emerges to be absent.

 

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