Differences between elementary and reticulate body:
Elementary body Reticulate body (Initial body)
Size about 0.3µm 0.5 µmRigid cell wall Fragile cell wallRelatively resistant to sonication Sensitive to sonicationResistant to trypsin Lysed by trypsinSubunit in cell envelope No sub unit in envelopeRNA: DNA content is 1:1 RNA: DNA is 3:1Toxic for mice Not toxic for miceInfectious Not infectiousAdapted for extracellular survival Adapted for intracellular growthStructure and chemical composition:
Test of highly purified materials reveals:Outer cell wall:
It looks like the cell wall of Gram negative bacteria, consists of relatively high lipid content.
It is firm and does not have typical bacterial peptido glycan. The tetra peptide related matrix is seen. Penicillin binding proteins take place in chlamydiae. Cell wall formation is inhibited by cycloserine and penicillins and substances which inhibit transpeptidation. Lysozyme has no effect on chlamydial cell wall and N-acetyl muramic acid emerges to be absent.
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