Direct Elisa:
In direct test solid phase (i.e., plastic plates) is sensitized with antigen. In another words antigen is coated in solid phase. It is washed to eliminate unattached antigens. The test antibody is added and permitted to react with the antigen and surplus un-reacted antibody is washed off. Then a ligand having enzyme which is added that binds to the antibody molecule.
The enzyme employed may be either alkaline phosphatase or Horse radish peroxidase. After washing, to eliminate the unattached ligand, the substrate for the enzyme employed is added. When the enzyme is attached via the ligand to antibody molecule, it will react and liberate a chromogenic compound (i.e., colored substance).
Figure: Indirect binding assay
The ultimate intensity of the color is read with the help of a spectrophotometer.
In indirect assay technique an antigen is coated onto the surface of polystyrene wells. The specimen having particular immunoglobulin for the suspected antigen is added followed by the enzyme labeled particular immunoglobulin (i.e., conjugate).In a positive condition, an invisible antibody-antigen complex is made. The complex is made visible by the addition of a substrate that the enzyme in the conjugate modifies to generate a color change. This reaction can be viewed by the naked eye or measured by electronic means like spectrophotometer.
In indirect technique the antibody to immunoglobulin is tagged with enzyme and not to the antibody which reacts with the antigen.
Latest technology based Biology Online Tutoring Assistance
Tutors, at the www.tutorsglobe.com, take pledge to provide full satisfaction and assurance in Immunology help via online tutoring. Students are getting 100% satisfaction by online tutors across the globe. Here you can get homework help for Immunology, project ideas and tutorials. We provide email based Immunology help. You can join us to ask queries 24x7 with live, experienced and qualified online tutors specialized in Immunology. Through Online Tutoring, you would be able to complete your homework or assignments at your home. Tutors at the TutorsGlobe are committed to provide the best quality online tutoring assistance for Microbiology Homework help and assignment help services. They use their experience, as they have solved thousands of the Biology assignments, which may help you to solve your complex issues of Immunology. TutorsGlobe assure for the best quality compliance to your homework. Compromise with quality is not in our dictionary. If we feel that we are not able to provide the homework help as per the deadline or given instruction by the student, we refund the money of the student without any delay.
tutorsglobe.com mineral nutrition assignment help-homework help by online plant physiology tutors
Introduction to Inorganic Chemistry tutorial all along with the key concepts of What is Inorganic Chemistry, Categorization of Inorganic Compounds, History of Inorganic Chemistry, Advances in inorganic chemistry, applications of Inorganic chemistry
www.tutorsglobe.com offers Factory Overheads homework help, assignment help, case study, writing homework help, online tutoring assistance by accounting tutors.
www.tutorsglobe.com offers alkenes reduction reactions, functional group reactions of reduction homework help, assignment help, online tutoring assistance, organic chemistry solutions by online qualified tutor's help.
Prokaryotic Cell Structure tutorial all along with the key concepts of Prokaryotic Cellular Components, Prokaryotic Cell Wall, Cytoplasmic Membrane, Nuclear Material, Cytosol, Ribosomes, Flagella, Bacterial chromosomes, Plasmids
Transport Systems tutorial all along with the key concepts of Transport Systems in plants, Xylem, Phloem and Transport system in Animals
tutorsglobe.com thalassemia assignment help-homework help by online genetic diseases tutors
coil pitch is the distance among the two active sides of similar coil under adjacent opposite poles. it is described in terms of number of slots each pole or electrical degrees.
tutorsglobe.com true cost-of-living adjustments assignment help-homework help by online cost-of-living adjustments tutors
concept of costs with the respective concepts of economies of scale, dis economies of scale, economies of scope, diseconomies of scope, direct cost and opportunity cost, Activity based costing, managerial economics, answering questions to managerial economics by tutors.
Theory and lecture notes of Probability Distributions all along with the key concepts of Probability Functions, Mean, Variance, Standard Deviation, Random Variable, Multinomial Distribution and Poisson Distribution. Tutorsglobe offers homework help, assignment help and tutor’s assistance on Probability Distributions.
tutorsglobe.com recombinant dna and molecular cloning assignment help-homework help by online genetic engineering tutors
Synthesis and Reactions of Coumarins tutorial all along with the key concepts of Physical and Chemical Properties of Coumarins, Reactions of Coumarins, Resonance structures of Coumarin and Synthesis of Coumarins
www.tutorsglobe.com offers assignment problem, hungarian method in operation research assignment help - free tutorials on introduction to assignment problem and algorithm for assignment problem.
The electronic home appliance that is employed to wash the several types of clothes without applying any physical efforts is termed as a Washing machine.
1961618
Questions Asked
3689
Tutors
1456237
Questions Answered
Start Excelling in your courses, Ask an Expert and get answers for your homework and assignments!!