--%>
Assignment Help - homework help

  Antigen Antibody Reactions

Antigen Antibody Reactions Immuno Fluorescence:

Fluorescence:

Fluorescence is a phenomenon stated as an emission of light of a specific color by a substance whenever it is irradiated with light of a different color.

Fluorochromes:

Substances that exhibit the phenomenon of fluorescence are termed as fluorochromes. Fluorochromes are organic substances acquired by synthesis or natural techniques.

There are lots of fluorochromes: For labeling antibodies the Fluorochrome must have the properties shown below:

1. Must have chemical group capable of making stable compounds with protein molecules (i.e., azo carbamide, sulfonamide, thiocarbamide,)

2. Fluorochromes which have not been coupled must be easy to eliminate.

3. Fluorescent light emitted must be influential & must refuse minimally.

4. Must not have chemical groups which in combination with a protein could disturb the reaction.

5. The fluorescent color of the conjugate must be dissimilar from that given off by normal cellular substrate (i.e., Autofluorescence)

6. The conjugate resultant from the coupling with the protein must be as stable as possible in normal situation of preservation.

7. Immunological reactivity must be retained.

8. Coupling technique must be simple

9. Fluorochrome must be easy to synthesize, easy to manipulate, economical and safe to those who manipulate them.

 

Latest technology based Biology Online Tutoring Assistance

Tutors, at the www.tutorsglobe.com, take pledge to provide full satisfaction and assurance in Immunology help via online tutoring. Students are getting 100% satisfaction by online tutors across the globe. Here you can get homework help for Immunology, project ideas and tutorials. We provide email based Immunology help. You can join us to ask queries 24x7 with live, experienced and qualified online tutors specialized in Immunology. Through Online Tutoring, you would be able to complete your homework or assignments at your home. Tutors at the TutorsGlobe are committed to provide the best quality online tutoring assistance for Microbiology Homework help and assignment help services. They use their experience, as they have solved thousands of the Biology assignments, which may help you to solve your complex issues of Immunology. TutorsGlobe assure for the best quality compliance to your homework. Compromise with quality is not in our dictionary. If we feel that we are not able to provide the homework help as per the deadline or given instruction by the student, we refund the money of the student without any delay.