Problem
I. What does ELISA stand for?
II. Where are antibodies naturally found?
III. How do doctors use the immune response to protect you from disease?
IV. Why is rapid detection of disease important?
V. Name one ELISA you can purchase over the counter at a pharmacy.
VI. Click on Figure 1 in the notebook of the virtual lab. What are the four steps of an ELISA protocol?
VII. In steps 3 and 4 of an ELISA plate. What has the ELISA plate been pretreated with and why?
VIII. Why do you need negative and positive control samples in this ELISA?
IX. A. Why is the plate washed in step 6?
X. What would happen if you didn't wash the plate thoroughly?
XI. In this ELISA, what reacts with the target antigen in a positive sample?
XII. In this ELISA, what reacts with the secondary antibody in a positive sample?
XIII. True or False: The positive results in an ELISA can be quantitatively measured.
XIV. Record your results in the table below. (+) for color change (-) for no color change
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Patient A
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Patient B
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Patient C
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+ Positive Control
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- Negative Control
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1:2
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1:10
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1:100
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XV. Did you complete the ELISA correctly? (yes/no) If not explain what went wrong.
XVI. What do the results indicate about:
Patient A:
Patient B:
Patient C: