Introduction:
Download the attached lab manual at the bottom of the page. Access "Experiment 7: Motility." Be sure to read the entire experiment and organize time, materials, and workspace before beginning. Remember to review the safety sections and wear goggles when working with chemicals.
Part 1: Direct Observation of Motility
This section gives the candidate additional practice in the preparation of wet mount slides and the observation of live organisms. Wet mount preparations of Escherichia coli and Staphylococcus epidermidis are evaluated for motility.
Part 2: Motility Tubes
In this section the candidates utilize motility tubes to confirm their motility observations of E. coli and S. epidermidis.
Note: You will use the CD-ROM's Virtual Microscope to complete the lab activities. No actual microscope will be needed to complete the lab activities.
Task:
Upon completion of the lab, write a brief essay (suggested length of 1-2 pages) in which you:
A. Describe the four classes of flagellar arrangement common in microbes.
B. Explain the purpose of inoculating with a needle instead of a loop in the motility test.
C. Discuss which of your microbes, if any, were positive for motility in the motility tube.
D. Discuss whether you were able to directly observe motility in the wet-mount preparations.
1. Describe which organisms were observed, if any.
E. When you use sources, include all in-text citations and references in APA format.