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define the agarose gel electrophoresis agethis technique is used to analyze serum proteins haemoglobin variants lactate dehydrogenase isoenzymes
define the gel electrophoresisgel electrophoresis is among the most powerful and yet conveniently used methods of macromolecular separation the gels
define about the paper electrophoresisin paper electrophoresis the sample is applied to a point on a strip of filter paper or cellulose acetate
define role of buffer in electrophoresisbuffer ions have a two-fold purpose in electrophoresis they carry the applied current and they fix the ph at
explain resonance effectsresonance effects the application of the resonance effect for predicting the relative rates of acid-catalyzed ester and
explain about the zone electrophoresiszone electrophoresis which was introduced about a decade later describes the migration of charged
define free solution or moving boundary methodthe first electrophoresis technique used in the study of protein was free solution or moving boundary
explain the compound decarboxylatesthe compound decarboxylates it is a disubstituted malonic acid in which the two a substituents are connected
explain about the electrophoresiselectrophoresis refers to the movement of charged particles because of an external electric field because charged
define efficiency - terminologies used in chromatographyefficiency in chromatography is expressed as number of theoretical
define selectivity - terminologies used in chromatographyselectivity is a measure of preference a stationary phase shows for one solute over another
define resolution - terminologies used in chromatographyresolution is the effectiveness of the separation and is determined by two factorsthese
distribution coefficient - terminologies used in chromatographyduring the purification or separation of the biomolecules it should be kept in mind
what is anionic intermediatethe spectrum is consistent with the formation of an anionic intermediate-a stable meisenheimer complex the dotted
define development - terminologies used in chromatographydevelopment is the process of passing a mobile phase over the adsorbent or support during
nucleophilic aromatic substitution reactionthe product is formed by a nucleophilic aromatic substitution reaction in which chloride is displaced by
define gas liquid chromatographythis is used for separating and identifying volatile compounds of fatty acids hydrocarbons sterols amino acids and
explain the un-ionized form of vanillinit is the un-ionized form of vanillin that has the typical odor in naoh solution the phenol group
define thin layer chromatographythis is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel alumina
valence electron of pdpd has ten valence electrons in the neutral atom there are no charges and no x-type ligands in the complex as this is a d10
why ascending method of paper chromatography is preferredthe ascending method is preferred because of the simplicity of the set up in actual method
define descending type of paper chromatographyin descending chromatography the movement or flow of mobile phase is in downward direction in this
define ascending type of paper chromatographythe ascending type consists in dipping the lower end of the paper containing the sample spots into the
explain following trems a state three structural features of prokaryotic cellsb describe the functions in eukaryotic cells of lysosomes ribosomes and
define technique of paper chromatographypaper chromatography is used as1ascending 2descending or3horizontal technique circular paper chromatographyit