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define essential parts of photocolorimeter - sample cuvettesfor accurate and precise reading cuvette must be transparent clean devoid of any
define essential parts of photocolorimeter - monochromatorsthis is a means of selecting a sufficiently narrow waveband early colorimeters used glass
define essential parts of photocolorimeter - light sourcethe light source is usually a tungsten lamp for wavelength in the visible range 320-700 nm
conjugate-base anion of diethyl malonatethe conjugate-base anion of diethyl malonate would be formed with sodium ethoxide but it would not react with
define instrument for absorbed radiation - spectrophotometerin contrast to colorimeter spectrophotometer is an instrument that is capable of
the ketone is more stable than its enolbecause the ketone is more stable than its enol more energy must be expended for it to ionize consequently
define instrument for measurement of absorbed radiation-colorimeterthe simplest types of photometric instruments are designed for measurements in
define instrument for measurement of absorbed radiationa photometer this is an instrument which it designed to measure the intensity of the beam of
define type of instruments used in spectral techniquesa wide range of instruments are available possessing a combination of features but there is no
calculate the concentration of unknown solution from standard curvei first take different volume of standard solution say 02 04 06 08 10 ml and then
define the beers law - nutritional biochemistrybeers law states that the concentration of a substance is directly proportional to the amount of
define the basic concepts behind the spectral techniquesenergy is transmitted via electromagnetic waves that are characterized by their frequency and
why the rate of ketone halogenation is independentbecause the rate of ketone halogenation is independent of the halogen concentration the rates of
define methods of quantitative analysis and colorimetrymany methods of quantitative analysis are based upon the production of coloured solutions in
define about the spectroscopy- colorimetryspectroscopy therefore is the study of the absorption and emission of radiation by matter absorption
define about the photometry - colorimetryphotometry is the measurement of the luminous intensity light or the amount of luminous light falling on a
define about the spectral techniques - colorimetrymany determinations in the biochemistry laboratory are based on measurements of radiant energy
define the disc electrophoresisdisc electrophoresis has improved resolution and strong bands are obtained it requires a two-gel system and several
explain about the gel bands detection techniquesgel bands resulting from a gel electrophoretic separation may be detected by staining radioactive
define polyacrylamide gel electrophoresis pageacrylamide gel has the advantage over starch in that it is easier to prepare and is more inert the pore
explain protonationprotonation of the ring double bond gives a tertiary carbocation that reacts with the carbonyl oxygen of the ester to give the
define the starch gel electrophoresisstarch gel has the advantage of fine texture and absence of protein adsorption with a molecular sieving effect
the reversible hydration of carbon dioxide the reversible hydration of carbon dioxide to carbonic acid is an acid-catalyzed addition of water that is
carboxylate salts carboxylate salts are less reactive than esters in nucleophilic acyl substitution reactions for two reasons first resonance
define about the cellulose acetate electrophoresis caeif the hydroxyl groups in cellulose are reacted with acetic anhydride cellulose is acetylated