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  Laboratory Diagnosis

Laboratory Diagnosis:

Specimens: Skin biopsy, intradermal aspirates, slit smear, Blood, and CSF are composed for examination.

Direct examinations:  Dark field microscopy and Giemsa staining are completed to explain the organisms.

Polymerase chain reaction (i.e., PCR): Blood, Buffy coat, Plasma, CSF, urine and Ticks are gathered. From such specimens DNA is extracted and employing Borrelia particular primers, the DNA is amplified and detected.

Culture: Specimens are inoculated in BSK medium, and the growth of the organisms is looked for.

Serology: Indirect immunofluorescence test and ELISA tests are completed: Four fold rises in titre of particular 1gM antibodies are explained. Western blot test is completed to confirm the diagnosis.

Treatment: Tetracyclines, minocycline, doxy cycline, are employed.

Penicillin: Amoxycillin with probenecid are also efficient.

Prophylaxis: Shun Tick Bite.

 

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