Culture development:
Natural isolates generally produce commercially significant products in very low concentration and hence every attempt is made to raise the productivity of the selected organism. Increased outcomes might be attained by optimizing the cultural medium and growth situation, though this approach will be restricted by the organism’s maximum capability to synthesis the product.
The potential productivity of the organisms is controlled by its genome and, hence, the genome should be modified to raise the potential outcome. The cultural needs of the modified organisms would then be examined to give conditions which would fully exploit the raised potential of the culture. Numerous attempts are made to beneficially modify the genome of the enhanced strain. Therefore, the procedure of strain improvement includes the genetic modification of the organism, followed by reappraisals of its cultural needs.Strain selection:
The most significant factor for the success of any fermentation industry is a choice of strain. The chosen strain must possess the following characters to attain the desired product in industry.
1) The strain must be high yielding 2. It must have stable biochemical characteristics. 3) It must not generate undesirable substances. 4) It must be simply cultivated on large scale by using cheapest raw materials.
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