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  How DNA is cut

How DNA is cut:

All bacteria generate at least one sort of restriction enzymes. They are supposed to assist the recombinant researchers to allow them to cut the DNA but to assist in the very survival of the bacterial species in opposition to the invading bacterial viruses.  The restriction enzymes could cut up and render harmless invading viral DNA. Restriction enzymes cleave DNA at very particular places together with its length. The restriction enzyme ECORI (E.coli Restriction Enzyme I) generated through the intestinal bacterium E. coli recognizes the following sequence.

Two DNA molecules along with sticky ends (ends which are staggered or uneven) tend to join with other molecules along with a complementary sequence of nucleotides in the ends.  With similar enzyme, DNA fragments are cut along with the matching sequence of nucleotides that complements with the sticky ends.

 

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