Question: You are trying to separate two forms of an acidic protein, its original form and a form that is phosphorylated (covalently modified with inorganic phosphate, PO42-) in three sites. You would therefore choose a(n) _____________ exchange column for optimal separation, and would expect the ______________ form of the protein to elute last with a higher concentration of salt? Need Assignment Help? a. anion; fully phosphorylated Explain your answer (one clear sentence is sufficient): b. anion; unphosphorylated c. cation; fully phosphorylated d. cation; unphosphorylated