Assignment Task: The strains are: Dcoq1, Dqcr8, Dcyc1, Dcox6, Datp14, Dcrd1, Dsod1, Dsod2, Dgsh1, Daco1, Dkgd2, Disa2, Dhap5.
Question 1: Describe the way in which loss of mitochondrial DNA may lead to the cellular and biochemical changes in cells which ultimately cause these strains to fail to produce ATP via the mitochondrial electron transport system and ATP synthase.
Question 2: Using your understanding of the strains used, the experimental design and observations explain what was the source of reducing power inside cells that led to dye reduction? Your answer may be broad in the first instance but also try to explain what the immediate source of reducing equivalents was that led to TTC reduction. Justify your answer.