Assignment Task: Another drug that acts as an antifungal is clotrimazole. It's belongs to the class of antifungal drugs called azoles. You may have encountered or heard of clotrimazole in antifungal preparations used to treat athlete's foot, and some forms of thrush. Clotrimazole in activates the Erg11p protein.
1. How does clotrimazole inhibit fungal growth?
2. Which organelle do you predict Erg11p is localised to?
3. Reflecting back on the TLC image of WT and Dcrd1 mutant why do predict the levels of certain phospholipids change?
4. Is there a phenotype you hypothesise Dcrd1 mutant cells would have compared to the wildtype strain?
5. Which organelle do you predict Crd1p is localised to?
6. Which organelle(s) or membranes would be mostly likely affected by the phospholipid changes in a Dcrd1 mutant?