Start Discovering Solved Questions and Your Course Assignments
TextBooks Included
Solved Assignments
Asked Questions
Answered Questions
define about the spectral techniques - colorimetrymany determinations in the biochemistry laboratory are based on measurements of radiant energy
define the disc electrophoresisdisc electrophoresis has improved resolution and strong bands are obtained it requires a two-gel system and several
explain about the gel bands detection techniquesgel bands resulting from a gel electrophoretic separation may be detected by staining radioactive
define polyacrylamide gel electrophoresis pageacrylamide gel has the advantage over starch in that it is easier to prepare and is more inert the pore
explain protonationprotonation of the ring double bond gives a tertiary carbocation that reacts with the carbonyl oxygen of the ester to give the
define the starch gel electrophoresisstarch gel has the advantage of fine texture and absence of protein adsorption with a molecular sieving effect
the reversible hydration of carbon dioxide the reversible hydration of carbon dioxide to carbonic acid is an acid-catalyzed addition of water that is
carboxylate salts carboxylate salts are less reactive than esters in nucleophilic acyl substitution reactions for two reasons first resonance
define about the cellulose acetate electrophoresis caeif the hydroxyl groups in cellulose are reacted with acetic anhydride cellulose is acetylated
define the agarose gel electrophoresis agethis technique is used to analyze serum proteins haemoglobin variants lactate dehydrogenase isoenzymes
define the gel electrophoresisgel electrophoresis is among the most powerful and yet conveniently used methods of macromolecular separation the gels
define about the paper electrophoresisin paper electrophoresis the sample is applied to a point on a strip of filter paper or cellulose acetate
define role of buffer in electrophoresisbuffer ions have a two-fold purpose in electrophoresis they carry the applied current and they fix the ph at
explain resonance effectsresonance effects the application of the resonance effect for predicting the relative rates of acid-catalyzed ester and
explain about the zone electrophoresiszone electrophoresis which was introduced about a decade later describes the migration of charged
define free solution or moving boundary methodthe first electrophoresis technique used in the study of protein was free solution or moving boundary
explain the compound decarboxylatesthe compound decarboxylates it is a disubstituted malonic acid in which the two a substituents are connected
explain about the electrophoresiselectrophoresis refers to the movement of charged particles because of an external electric field because charged
define efficiency - terminologies used in chromatographyefficiency in chromatography is expressed as number of theoretical
define selectivity - terminologies used in chromatographyselectivity is a measure of preference a stationary phase shows for one solute over another
define resolution - terminologies used in chromatographyresolution is the effectiveness of the separation and is determined by two factorsthese
distribution coefficient - terminologies used in chromatographyduring the purification or separation of the biomolecules it should be kept in mind
what is anionic intermediatethe spectrum is consistent with the formation of an anionic intermediate-a stable meisenheimer complex the dotted
define development - terminologies used in chromatographydevelopment is the process of passing a mobile phase over the adsorbent or support during
nucleophilic aromatic substitution reactionthe product is formed by a nucleophilic aromatic substitution reaction in which chloride is displaced by