Define the Agarose gel electrophoresis (AGE)?
This technique is used to analyze serum proteins, haemoglobin variants, lactate dehydrogenase isoenzymes, lipoprotein fractions and other substances. The advantages of agarose gel include its lower affinity for proteins and its native clarity after drying which permits excellent densitometric examination. Usually 0.5 to 1.0 g of agarose per decilitre of buffer provides a gel of desired strength and with good migration properties. The sample volume required for AGE is relatively less (0.6-3/ul), and electrophoresis time is relatively short (30-90 minutes depending on experimental conditions).