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  Bacterial Culture

Bacterial Culture:

All bacteria require nutrients for their development. They require a nutrient medium for their augmentation and culture. The bacteriological medium is composed of identified chemical compounds. It is termed as chemically defined medium or synthetic medium. The compounds are proteins, lipids, carbohydrates, nucleic acids, vitamins and other complex organic substances. Certain complex materials involve peptones, meat extract, yeast extract and so on. The medium with the above raw materials can sustain the growth of a wide range of heterotrophic bacteria. Agar is involved as a non-nutritive solidifying agent. Nutrient agar and nutrient broth are simple solid and liquid media for the growth of heterotrophs correspondingly.

The preparation of bacteriological media includes the steps as follows:

1. Each ingredient or the absolute dehydrated medium is dissolved in the suitable volume of distilled water.

2. The pH of medium is established.

3. Agar is added and the medium (i.e., solid medium) is boiled to dissolve agar.

4. The medium is dispersed in tubes or flasks.

5. The medium is uncontaminated by autoclaving.

The augmentation of bacteria also need in addition to nutrients, physical conditions like temperature, gaseous conditions and pH (i.e., acidity and alkalinity) and other miscellaneous needs like illumination, hydrostatic pressure, and so on.

 

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