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  Clinical Manifestations of Vibrio

Clinical Manifestations:

Unforced vomiting and profuse watery diarrhea are the features of cholera. The quick dehydration and hypovolaemic shock might cause death in 12-24 hrs. The watery stool is colorless with flecks of mucus and distinctive fishy odor and also termed as ‘rice water stool’.

It has little protein and is distinct from the mucopurulent blood-stained stools of bacillary dysentery. Anuria and muscle cramps take place and the patient becomes weak and lethargic with low blood pressure and not present or has thready pulse.

Laboratory Diagnosis:

Stool specimens are gathered and inoculated into alkaline peptone water. Vibrios develop faster than other organisms. Subsequent to incubation for 3 to 6 hours, a loopful from the surface is inoculated on TCBS [i.e., Thiosulphate Citrate Bile salt Sucrose] agar. At this medium V. cholerae strains emerge as yellow, sucrose fermenting colonies. The colonies must be checked for the enzyme oxidase and then biochemical tests are completed. Confirmation of V. cholerae is completed with rabbit antibodies particular for O1 antigen. Whenever agglutination takes place, it is verified.

 

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