Anaerobic phase:
In the nonexistence of O2, at glyceraldehydes 3-phosphate dehydrogenase stage reoxidation of NADH cannot occur in respiratory chain. But the cells comprise limited coenzyme. Therefore to carry on the glycolysis NADH has to be reoxidized to NAD+. This is acquired through reoxidation of NADH via conversionof pyruvate to lactate (without generating ATP).
It is to be noticed that in the reaction catalyzed via glyceraldehydes 3-phosphate dehydrogenase, hence no ATP is generated.
Within the anaerobic phase oxidation of one glucose molecule generates
4-2=2 ATP.
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