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Calculate the pH at 0, 10.0, 25.0, 50.0, and 60.0 mL of titrant in the titration of 50.0 mL of 0.100 M NH3 with 0.100 M HCl.
A 25.0 ml sample of an aqueous solution of pure benzoic acid is titrated using standardized 0.150 M NaOH
A 30 mL sample of 0.35 M of lactic acid is titrated with a 0.25 M NaOH solution. Calculate the pH before NaOH is added.
The 125 mL flask can be wet when the 25 mL of seawater is added using the volumetric pipet. Why doesn't the water in the flask cause problems in the calculation
If potassium permanganate had been used as the titrant instead of cerium(IV), what would the balanced overall reaction be?
Using the following pKb (pKb = 4.11) of an unknown base, calculate the Ka of the corresponding conjugate acid
Caffeine is soluble in ethyl acetate. Do you think that the purity of your product could be checked by TLC using ethyl acetate as an elution solvent? Explain.
Sketch the expected chromatographic elution profile (protein concentration vs elution volume) if a mixture of these proteins is applied to a weak anion exchange
The first amino acid to be detected as a phenylthiohydantoin derivative on Edman degradation of the peptide was glutamic acid.
If the active site of a dipeptidase contains a glutamic acid residue (pKa 3.3) and a histidine residue (pKa 6.7).
Based on predicted pI (for example, a pI for turnip peroxidase is 9.10), how can you use DEAE-sepharose to further purify peroxidase?
The advantage of treating separate samples of a protein with two or more enzymes when sequencing a protein is that the products.
The TLC sheet is coated with highly polar silica gel and the solvent has a much lower polarity. Explain the relative Rf values on a structural basis.
Nothing happened for the 1:1 and the methanol. I did repeatedly place the sample on the spot, but I don't understand what went wrong?
Caffeine is soluble is ethyl acetate. Do you think that the purity of your product could be checked by TLC using ethyl acetate as an elution solvent? Explain.
To find the Ce4+ content in a solid, 4.37 g were dissolved and treated with and excess of iodate to precipitate Ce(IO3)4.
Conduct HPLC analysis of myoglobin and hemoglobin using a C18 and C4 column. Both proteins were in a buffer solution only.
A mixture of organic acids was separated by reversed-phase liquid chromatography. The data obtained for the chart recording were as follows.
How many extracts would be required to remove all but 10,000 molecules of X from the sample with repeated 10.00 mL portions of extract?
For HCB, log Kow = 8.0. What would be the predicted concentration of HCB due to bioconcentration in the fat of fish that swim in waters containing 0.000010 ppm
There is a danger of contaminating groundwater sources especially in unlined landfills since the leachate could reach the water table.
Consider a lake with 100 X 10^6 m^2 of surface area for which the only source of phosphorus is the effluent from a wastewater treatment plant.
BOD5 is the total amount of oxygen consumed by microorganisms during the first five days of biodegradation.
How would you calculate the total iron concentration from these concentrations? What if you wanted to know the iron-oxide concentration.
Steponaitis, Blackman, and Neff (American Antiquity 61:555-572, 1996) use the following correction for bulk elemental concentrations.