Question: Protein unfolding is highly cooperative. What is the nature of the protein at the mid-point of this transition? 50% of the protein molecules in the population are 50% folded. 50% of the protein molecules in the population are 100% folded. 100% of the protein molecules in the population are 50% folded. 100% of the protein molecules in the population are 100% folded. Need Assignment Help?