1. What happens if the spots are made too large when preparing a TLC plate for development?
2. What happens if the spots are made too small when preparing a TLC plate for development?
3. Why must the spots be above the level of the development solvent in the developing chamber?
4. What would happen if the spotting line and positions were marked on the plate with a ball-point pen?
5. Is it possible to distinguish two spots that have the same Rf value but represent different compounds? Give two different methods.
6. Name some advantages of using acetaminophen (Tylenol) instead of aspirin as an analgesic.