Problem:
Question: Which of the following best describes the way in which you would use restriction enzymes as you engineered bacterial cells to produce human proteins?
A) Restriction enzymes would be used only to cleave the vector DNA.
B) The same restriction enzyme would be used to cut both the donor DNA and the vector DNA.
C) One restriction enzyme would be used to probe the protein-coding region and another would be used to cleave that region from the larger DNA molecule.
D) Restriction enzymes would be used only to cleave the donor DNA.
E) One restriction enzyme would be used to cut the donor DNA and a different one would be used to cleave the vector DNA.
Provide the correct answer and justification for your choice.