learning
1. Name the two steps necessary for gene expression to occur. What are the products of these two steps?
2. Which of the products that you listed in the answer to question 1 might be considered intermediate product?
3. What does it means when I said that is single strand of DNA is considered the sense (or coding)?
4. Give definitions for the following 3 terms:
Silent mutation
Missense mutation
Nonsense mutation
given sequence the DNA
sense strand (coding strand)
In the space provide below:
a. write the DNA complement to this sequence.
b. Write what the mRNA sequence would be
c. Indicate the start codon. What would the anti-codon be? Which type RNA contains the anti-codon?
d. Using the genetic code provided write the amino acids that will result from this mRNA.
e. What would happen if the third A in the DNA sense strand was mutated to a T?
GCCTATGCCGCAGCTGAAG
a.
b.
c.
d.
e.
2nd
________ 2nd base in codon____________________________________
U C A G
-----------------------------------------------------------------
Phe Ser Tyr Cys U
Phe Ser Tyr Cys C
U Leu Ser STOP STOP A
Leu Ser STOP Trp G
-------------------------------------------------------------
Leu Pro His Arg U
Leu Pro His Arg C
C Leu Pro Gln Arg A
Leu Pro Gln Arg G
-------------------------------------------------------------
Lle Thr Asn Ser U
A Lle Thr Asn Ser C
Lle Thr Lys Arg A
Met Thr Lys Arg G
-------------------------------------------------------------
Val Ala Asp Gly U
Val Ala Asp Gly C
G Val Ala Glu Gly A
Val Ala Glu Gly G
_____________________________________________
1st base 3rd in codon
in codon