Tissue Engineering (bone):
I need an introduction to tissue engineering (bone) up to 500 to 800 words. Please, write the full references (Harvard system) in other separate pages.
Overall objectives Prepare porous gelatine-GAG scaffolds using freeze-drying fabrication and determine the effect of cross-linking density on (i) the mechanical properties and (ii) the viability of seeded MC3T3-E1 osteoblast cells. Establish whether Gelatin-GAG scaffolds might be suitable in a bone tissue engineering strategy.
Introduction should address a number of important points
i. Establishing the existing state of knowledge in the field by citing important and relevant literature
ii. Identifying a gap in the current literature, thus outlining a clear need/motivation for the study undertaken
iii. Identify the specific focus of your work by outlining specific objectives/hypotheses under investigation.
Structure of Introduction First paragraph Broad information on the topic and some important/relevant background information and literature. Second/Third paragraphs
Progressively narrower the focus of the study, and begin to identify a potential gap in the literature Begin to outline the need/motivation for the study (However, it is not known…/However, the precise mechanism) Final paragraph Clearly state either objectives/hypothesis under investigation Briefly outline of how these are going to be investigated.
Introduction should address:
Natural biomaterials as scaffolds in bone tissue engineering
Use of Gelatin instead of Collagen (widely established) as a scaffold biomaterial Structural differences Possible advantages of using Gelatin Has it been used in other tissue regeneration strategies?
Freeze-drying preparation Brief background and what makes it useful in Tissue Engineering?
Clear statement of objectives and a brief outline of how the study will be carried out.