Epidemiology at CDC in Atlanta, GA have called you in examine and increased incidence of the leukemia in a remote region of Africa. Shortly after arriving, an RNA virus was separated from patients and found to induce leukemia in mice. At this point you don't know the mechanism or viral gene product associated with developed of leukemia. One of your lab assistant has isolated a variant of virus and reports that this variant is missing portion of (20%) of the genome of original virus.
Furthermore, this variant doesn’t induce leukemia in mice.
a) How would you determine which portion of genome is associated with development of leukemia?
b) How would you determine the viral mechanism and product associate with development of leukemia?