Illustrate between normal phase and reverse phase hplc

Question

Question 1: A semiconductor exhibits a band gap of 2.50 eV. Which wavelength of radiation is required to excite electrons to the conduction band?

Question 2: What is the general elution problem? Describe why an elution gradient in HPLC can address this issue?

Question 3: Describe between normal phase and reverse phase HPLC? Which method would you choose for the separation of alcohols?

Question 4: Why does the pH affect separations of amino acids by electrophoresis? The electrode reactions in electrophoresis affect pH. How is this influence on separation addressed in an electrophoresis experiment?

Question 5: In Potentiometric experiment, Ag/AgCl electrode is immersed in a solution containing an unknown amount of Cl- ions. A potential of 0.205 V is measured between this working electrode and a Ag/Agcl reference electrode in solution of 1M Cl- concentration. (T=298.15^oC)

Question 6: Based on a lattice parameter a=4.0 Å and x-ray wavelength 1.2 Å, calculate the diffraction angle θ for the (1 0 0) plane.

Question 7: Describe the purpose and physical principle of a lens within an electron microscope.

Question 8: You need to determine the oxidation state of iron in an unknown solid iron compound.

Question 9: For diprotic acid H₂A with pK_a1 = 2.4, pK_a2 = 7.6 calculate the approximate concentrations of each spices (H₂A, HA^-, A^2-) at a pH = 4.0. If their total concentration is 1.0 M.

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