How do researchers use these enzymes, Biology

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How do researchers use these enzymes

Problem

1) Ligation

a) What do these enzymes do? How do they work?
b) How do researchers use these enzymes?
c) Why do we need to heat the digests up to 65^oC prior to setting up the ligation?

2) What is a recombinant DNA molecule?

a) What is a gene of interest?
b) What is OUR gene of interest for our project?

i) Would the gene you listed usually be used as a gene of interest by researchers? Why or why not?

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