Problem:
Given that some transcription factors such as AP1 bind multiple regulatory elements in many genes, how is specificity achieved? For example, why do only muscle cells make their specialized contractile proteins and only skin cells make keratin intermediate filaments?
Question 1: How do cells regulate transcription factor binding sites availability in the genome?
Question 2: How might you find which genes are regulated by a particular transcription factor?
Question 3: How might you find a particular transcription factor's binding sites in the promoter region of a gene?
Question 4: What type of DNA do you expect to find in a Chromatin IP using and antibody against acetylated Histone H3 Lysine 9? Methylated Histone H3 Lysine 9? Histone H2A? RNA pol II?
Question 5: How can just a few thousand transcription factors regulate all 25,000 human genes?
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