Gel chromatography is used to assess the extent of the, Chemistry

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Gel chromatography is used to assess the extent of the

An interesting enzyme (56 kDa) is used in catalytic amounts (=very small) to cleave a membrane protein (65 kDa) into two fragments, 43 kDa and 22 kDa. Gel chromatography is used to assess the extent of the reaction at various times. Three major fractions of proteins are eluted at each time. What are they and in what order are they eluted?

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