1. Explain what volume of concentrated HCl and what weight of tris(hydroxymethyl)amino methane(as the base) are needed to make the 100 ml of 0.25M buffer, pH 8.0?
2. Discuss how to prepare 250ml of 0.6 Mtriethanolamine buffer, pH 7.2, from the free amine and concentrated HCl ? (pKa for the amine hydrochloride is 7.8)