Determine the origin of a miniscule amount of blood


Determine the origin of a miniscule amount of blood found at a crime scene. To be certain, statistically speaking, beyond a reasonable doubt of the identity of the person, the blood sample should be subjected to DNA fingerprinting...
Answer choices 
1. One can never determine beyond a reasonable doubt with only small amounts of DNA. 

2. using numerous restriction enzymes sequentially to cut non-coding regions with resulting fragments subjected to subsequent RFLP analysis.

3. using PCR analysis with numerous primer sets to distinct VNTR loci or areas with known single nucleotide polymorphisms (SNPs), particularly in non-coding regions of the genome.
4. using numerous restriction enzymes simultaneously to cut coding regions of the genome common to all people with resulting fragments subjected to subsequent RFLP analysis.

5. using PCR analysis with numerous primer sets to genes common to all people (e.g., coding regions like 18s rRNA genes, DNA polymerase, hemoglobin, etc.). 

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Biology: Determine the origin of a miniscule amount of blood
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