Determine the change in fret pair separation distance


The fluorescence intensity fluctuates among the 10,000 and 5000 counts per second in a FRET experiment designed to monitor conformational changes in T$ lysozyme. Assuming that 7500 counts represents a FRET efficiency of 0.5; determine the change in FRET pair separation distance during the reaction?

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Chemistry: Determine the change in fret pair separation distance
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