Problem:
Question 1: You want to perform an interrupted-mating mapping with an E.coli Hft strain taht is Pyr+, Met+, Xyl+, Try+, Arg+, His+, Mal+, and Str^(5). Describe an appropriate bacterial strain to be used as the other partner in this mating.
Question 2: In an HfrxF(-) cross, the pyrE gene enters the recipient in 5 minutes, but as this time point there are no exconjugants that are Met^(+) , Xyl^(+), Tyr^(+), Arg^(+), His^(+), or Mal^(+). The mating is now allowed to proceed for 30 minutes and Pyr^(+) exconjugants are selected. Of the Pyr^(+) cells, 32% are Met^(+), 94% are Xyl^(+), 7% are Tyr^(+), 59% are Arg^(+), 0% are His^(+),and 71% are Mal^(+). What can you conclude about the order of the genes?
Show your calculations, but you do not need to write out any explanation.