Computation of transformation efficiency, Biology

+44 141 628 6080
[email protected]

Computation of transformation efficiency

I want help with this transformation efficiency problem:

You add up 10 ng of pGEM to 50 ul of competent cells, and then dilute this into a total of 1 ml Luria broth after the transformation. You take 100 ul of the Luria broth and plate on and agar plate. The next day, there are 780 colonies on the plate. Determine the transformation efficiency?

Request for Solution File

Ask an Expert for Answer!!

Biology: Computation of transformation efficiency

Reference No:- TGS037712

Expected delivery within 24 Hours

Have a Question? (oR Write a Review)

Write atleast 100 words!!

Recent Questions Asked Biology

Q : Explain methodologies used for requirements elicitation

Why does project planning generally come before analysis and design? Write down some of the methodologies generally used for requirements elicitation?

Q : E-business environment and marketing system

Please discuss how relationships are created and enhanced in e-business environment. Critically discuss the potential risks of using Web 2.0 tools. Provide several examples.

Q : Disadvantages to forming strategic alliance

Critically discuss the issues, challenges, or disadvantages to forming a strategic alliance (focus on supply chain).

Q : Cloning of a dna fragment

Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.

Q : Computation of transformation efficiency

You add up 10 ng of pGEM to 50 ul of competent cells, and then dilute this into a total of 1 ml Luria broth after the transformation. You take 100 ul of the Luria broth and plate on and agar plate. The next day, there are 780 colonies on the plate

Q : Elements in conducting technology alternatives cost analysis

Write down the most significant elements in conducting a technology alternatives cost analysis? Why is it significant to perform ROI evaluation of new technologies?

Q : Significance of restriction endonucleases

Restriction endonucleases are enzymes that digest the DNA. They are not similar digestive enzymes that completely digest DNA to monomers so cells can eat nucleic acids.

Q : Ebay supply and demand

How many natural diamonds are for sale at moment? Note the wide array of sizes and prices of the diamonds.

Q : Clostridium and streptococcus

Clostridium and Streptococcus are both catalase-negative. Streptococcus grows through fermentation. Explain why is Clostridium killed by oxygen, while Streptococcus is not?

1929755

Questions
Asked

3,689

Active Tutors

1416658

Questions
Answered

Start Excelling in your courses, Ask a tutor for help and get answers for your problems !!

ask Question

Asked Questions

How are schools important in the struggle for power

Question: How are schools important in the struggle for power and status?

Compare critical feminist theory and power-control theory

Compose a paper that compares and contrasts critical feminist theory and power-control theory as forms of gendered criminology. Your paper should:

Statement about political systems controlled by men

Multiple Choice Question Identify a true statement about political systems controlled by men. Multiple choice question.

What is meant by indigenismo

Question: What is meant by indigenismo (Keywords in Week 2)? What is las cronicas de castas (Cope)?

How is it rational for the owner of a factory or plantation

How is it rational for the owner of a factory or plantation to work his laborers to death or disability when they can easily be replaced cheaply?

Symbolic interactionism took off as a theoretical approach

Question: Symbolic interactionism took off as a theoretical approach to the study of deviance during the 1960s

Cases of local communities using modern technology

Question: Cases of local communities using modern technology to preserve and revive their traditions: