Cloning of a dna fragment
1) Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.
2) Explain the use of an expression vector to isolate the gene from the cDNA library which codes for a precise protein.
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One of these dogs ate some of a birthday cake. Digestion and electrophoresis of DNA found at the scene of the crime with the restriction enzyme Scs1 revealed a banding pattern included of four bands. Which dog was most probable not responsible for
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Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.
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Restriction endonucleases are enzymes that digest the DNA. They are not similar digestive enzymes that completely digest DNA to monomers so cells can eat nucleic acids.
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