Cloning of a dna fragment
1) Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.
2) Explain the use of an expression vector to isolate the gene from the cDNA library which codes for a precise protein.
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Explain DNA sequencing with reference to the cloning of a DNA fragment in bacteriophages M13 based cloning vector.
You add up 10 ng of pGEM to 50 ul of competent cells, and then dilute this into a total of 1 ml Luria broth after the transformation. You take 100 ul of the Luria broth and plate on and agar plate. The next day, there are 780 colonies on the plate
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Restriction endonucleases are enzymes that digest the DNA. They are not similar digestive enzymes that completely digest DNA to monomers so cells can eat nucleic acids.
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