Discussion:
Calculation of Transformation Efficiency
I need help with this transformation efficiency problem:
You add 10 ng of pGEM to 50 ul of competent cells, and then dilute this into a total of 1 ml Luria broth after the transformation. You take 100 ul of the Luria broth and plate on and agar plate. The next day, there are 780 colonies on the plate. What was the transformation efficiency?
Write your answer/response in detail with examples and facts and figures using APA style of formatting, size 12, font Times new roman and answer must be single spaced