Problem: Debug has already successfully overcome several technical challenges. They developed reliable mass-rearing systems, automated sex-sorting tools with extremely low female contamination rates, and designing release strategies that adapt to seasonal and environmental conditions (Debug, 2025). However, the seminar also highlighted the challenges that remain. Scaling the system to larger regions or areas with fewer resources is complex, especially since continuous mosquito releases and long-term monitoring are required. Migration of wild mosquitoes from untreated zones can quickly repopulate suppressed areas, raising important strategic questions about the minimum effective deployment size. There are also scientific uncertainties, including how evolutionary pressures might influence Wolbachia compatibility over time or how genetic diversity in wild mosquito populations could affect release outcomes (Debug, 2025). Need Assignment Help?