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define the beers law - nutritional biochemistrybeers law states that the concentration of a substance is directly proportional to the amount of
define the basic concepts behind the spectral techniquesenergy is transmitted via electromagnetic waves that are characterized by their frequency and
define methods of quantitative analysis and colorimetrymany methods of quantitative analysis are based upon the production of coloured solutions in
define about the spectroscopy- colorimetryspectroscopy therefore is the study of the absorption and emission of radiation by matter absorption
define about the photometry - colorimetryphotometry is the measurement of the luminous intensity light or the amount of luminous light falling on a
define about the spectral techniques - colorimetrymany determinations in the biochemistry laboratory are based on measurements of radiant energy
define the disc electrophoresisdisc electrophoresis has improved resolution and strong bands are obtained it requires a two-gel system and several
explain about the gel bands detection techniquesgel bands resulting from a gel electrophoretic separation may be detected by staining radioactive
define polyacrylamide gel electrophoresis pageacrylamide gel has the advantage over starch in that it is easier to prepare and is more inert the pore
define the starch gel electrophoresisstarch gel has the advantage of fine texture and absence of protein adsorption with a molecular sieving effect
define about the cellulose acetate electrophoresis caeif the hydroxyl groups in cellulose are reacted with acetic anhydride cellulose is acetylated
define the agarose gel electrophoresis agethis technique is used to analyze serum proteins haemoglobin variants lactate dehydrogenase isoenzymes
define the gel electrophoresisgel electrophoresis is among the most powerful and yet conveniently used methods of macromolecular separation the gels
define about the paper electrophoresisin paper electrophoresis the sample is applied to a point on a strip of filter paper or cellulose acetate
define role of buffer in electrophoresisbuffer ions have a two-fold purpose in electrophoresis they carry the applied current and they fix the ph at
explain about the zone electrophoresiszone electrophoresis which was introduced about a decade later describes the migration of charged
define free solution or moving boundary methodthe first electrophoresis technique used in the study of protein was free solution or moving boundary
explain about the electrophoresiselectrophoresis refers to the movement of charged particles because of an external electric field because charged
define efficiency - terminologies used in chromatographyefficiency in chromatography is expressed as number of theoretical
define selectivity - terminologies used in chromatographyselectivity is a measure of preference a stationary phase shows for one solute over another
define resolution - terminologies used in chromatographyresolution is the effectiveness of the separation and is determined by two factorsthese
distribution coefficient - terminologies used in chromatographyduring the purification or separation of the biomolecules it should be kept in mind
define development - terminologies used in chromatographydevelopment is the process of passing a mobile phase over the adsorbent or support during
define gas liquid chromatographythis is used for separating and identifying volatile compounds of fatty acids hydrocarbons sterols amino acids and
define thin layer chromatographythis is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel alumina