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explain the types of double beam systemsfollowing are types of double beam systemsa dual beam in space typeb dual beam in time typein type a separate
define single beam and double beam optical pathin simpler instruments a single beam of light passes through the cuvettes and reaches the detector
define essential parts of photocolorimeter - sample cuvettesfor accurate and precise reading cuvette must be transparent clean devoid of any
define essential parts of photocolorimeter - monochromatorsthis is a means of selecting a sufficiently narrow waveband early colorimeters used glass
define essential parts of photocolorimeter - light sourcethe light source is usually a tungsten lamp for wavelength in the visible range 320-700 nm
define instrument for absorbed radiation - spectrophotometerin contrast to colorimeter spectrophotometer is an instrument that is capable of
define instrument for measurement of absorbed radiation-colorimeterthe simplest types of photometric instruments are designed for measurements in
define instrument for measurement of absorbed radiationa photometer this is an instrument which it designed to measure the intensity of the beam of
define type of instruments used in spectral techniquesa wide range of instruments are available possessing a combination of features but there is no
calculate the concentration of unknown solution from standard curvei first take different volume of standard solution say 02 04 06 08 10 ml and then
define the beers law - nutritional biochemistrybeers law states that the concentration of a substance is directly proportional to the amount of
define the basic concepts behind the spectral techniquesenergy is transmitted via electromagnetic waves that are characterized by their frequency and
define methods of quantitative analysis and colorimetrymany methods of quantitative analysis are based upon the production of coloured solutions in
define about the spectroscopy- colorimetryspectroscopy therefore is the study of the absorption and emission of radiation by matter absorption
define about the photometry - colorimetryphotometry is the measurement of the luminous intensity light or the amount of luminous light falling on a
define about the spectral techniques - colorimetrymany determinations in the biochemistry laboratory are based on measurements of radiant energy
define the disc electrophoresisdisc electrophoresis has improved resolution and strong bands are obtained it requires a two-gel system and several
explain about the gel bands detection techniquesgel bands resulting from a gel electrophoretic separation may be detected by staining radioactive
define polyacrylamide gel electrophoresis pageacrylamide gel has the advantage over starch in that it is easier to prepare and is more inert the pore
define the starch gel electrophoresisstarch gel has the advantage of fine texture and absence of protein adsorption with a molecular sieving effect
define about the cellulose acetate electrophoresis caeif the hydroxyl groups in cellulose are reacted with acetic anhydride cellulose is acetylated
define the agarose gel electrophoresis agethis technique is used to analyze serum proteins haemoglobin variants lactate dehydrogenase isoenzymes
define the gel electrophoresisgel electrophoresis is among the most powerful and yet conveniently used methods of macromolecular separation the gels
define about the paper electrophoresisin paper electrophoresis the sample is applied to a point on a strip of filter paper or cellulose acetate
define role of buffer in electrophoresisbuffer ions have a two-fold purpose in electrophoresis they carry the applied current and they fix the ph at