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How can I improve my Gel Extraction yields. We use the standard protocol from Qiagen, gel extraction, dissolve in QG buffer at 42C and purify via anion exchange columns.
A sex-linked recessive allele of a gene "c" produces red-green colour blindness in humans. A normal woman whose mother was colour-blind, marries a colour-blind man.
This is reference to a review on C. elegans mosaic analysis by Yochem and Herman, in which the authors make a distinction between free chromosome fragments and extrachromosomal arrays.
I know of melanopsin, a photo pigment in mammal eye that helps regulate circadian rhythms. It responds most strongly to blue spectrum light. There have been experiments that demonstrated that intens
I was always curious that all the animals can swim in water. They don't need any training or to learn swimming.
My goal is to sequence the active site of some enzymes I'm interested in, but I'm not sure how to go about designing PCR primers for a specific site on my protein. Additionally, the active site is o
I know that electroporation is widely used for introducing genes into cells, but running a full transfection experiment is not so feasible in the lab I am working in.
(Exogenous) melatonin is administered at night for treatment of circadian rhythm disorders, but melatonin taken during the day is linked with depression.
In my lab we've observed a phenomenon in which a culture of E. coli is found to shift from normal rod growth to filamentous growth and then back to normal rod growth again several times over the cou
What is the chance that a color-blind male and a carrier female will produce
I have noticed that some of my sporadic gray hairs are gray at the tip side but oddly, not near the roots. Some are even only gray in the middle. I find all of this very counter intuitive, and I as
Carcharodon carcharias the great white shark has forty three pairs of chromosomes; its gametic cells contain _____ DNA double helices.
He explained that the reason why you need to get more good proteins when you are sick is that the body is building antibodies by the adaptive immune system and later for innate immunity.
I ask this question because if Darwin's theory of evolution is true then foods like vegetables that us human beings have been eating and attaining better health from eating for millions of years sho
I am trying to convert a set of Affymetrix ID's, like this one 143053_at_3745, to Flybase Gene Numbers (FBgn) like this one FBgn0000015. I have downloaded the Flybase file required to do so (as des
Cholesterol is transported in the bloodstream in lipoprotein particles. There are several classes of lipoprotein particles, but one of the most important classes is called Low-density lipoproteins (
I'm reading a journal article about mitochondrial protein import and it mentions that one of the mutant proteins tested showed background binding with another protein.
I am currently taking a biology class. I do not understand this concept. I understand that the electrostatic repulsion of the negative charges, resonance stabilization and hydration stabilization al
What I don't understand is that the authors seem to say that increasing sialylation might be a good thing to prevent cancer progression since it inhibits EGFR dimerization.
I want to choose a month to map these vegetation types that will allow the most accurate mapping of their distribution of vegetation across the mudflat.
Every time I make fajitas, I cut into bell and chili peppers and notice how hollow they are. I always think to myself: what is the gas inside this pepper, and how does it get there?
There have been many times where I have an itch on say my arm and I scratch it, only to feel the scratching elsewhere on my body.
Typically we determine the concentration of proteins using a 280 nm reading. However, it is reasonable to use 205 nm. I was curious about the effectiveness of this method.
I am performing a gel extraction to purify DNA after a double digest with EcoRI and BamHI. After the gel extraction I need to complete a ligation step before bacterial transformation.
There is a lot of talk about inhumane treatment of animals and how we don't kill them in the proper way. I wonder how much research is done on this subject.