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  Regeneration of RuBP

Regeneration of RuBP:

The glyceraldehydes 3-phosphate molecules are changed to RuBP by a series of reactions that produce 4C, 6C and 7C phosphorylated compounds like intermediates. For better and simple understanding of these types of reactions, a simplify scheme of Calvin cycle referring three CO2 molecules fixation reactions is shown below.

The reactions of regeneration of RuBP are like this:

1. A number of of the Glyceraldehyde 3-phosphate molecules are converted to dihydroxy acetone phosphates.

2. Glyceraldehyde 3-phosphate combines along with dihydroxy acetone phosphate to create fructose1, 6-bisphosphate.

3. Fructose 1, 6-bisphosphate goes through dephosphorylation to create fructose 6-phosphate.

4. Fructose 6-phosphate combines along with glyceraldehyde 3-phosphate acquired from the fixation of second molecule of CO2 to form Ribose 5-phosphate (R5P) and Erythrose 4-phosphate (Er4P).

5. Erythrose 4-phosphate combines along with DHAP acquired from the second CO2 fixation, to form sedoheptulose 1, 7-bisphosphate.

6. Sedoheptulose 1, 7-bisphosphate goes through dephosphorylation to creates sedoheptulose 7-phosphate.

7. Sedoheptulose 7-phosphate combines along with glyceraldehyde 3-phosphate acquired through the third CO2 fixation, to form two molecules of 5C compounds – ribose 5-phosphate and xylulose 5-phosphate (Xy5P).

8. xylulose 5-phosphate and Ribose 5-phosphate molecules are transformed to ribulose 5-phosphate (Ru5P).

9. After that Ru5P molecules are phosphorylated through ATP to form RuBP molecules that again enter into the cycle of CO2 fixation.

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In the above demonstration, three CO2 molecules are set and the net gain is a 3C termed as DHAP. These triose phosphate molecules merge to create hexose phosphates that are employed to create sucrose. For each carbon fixation 3ATP and 2 NADPH2 are consumed.

 

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