The PGA’s two molecules are further decreased to glyceraldehyde-3- phosphates in 2 steps. 1st, two PGA molecules are converted to 1, 3 -bisphosphoglyceric acids through the enzyme PGA kinase. This reaction takes two molecules of ATP in the ratio of one ATP for every molecule of 1, 3-bisphosphoglyceric acid created.
In the 2nd step, the two molecules of 1, 3-bisphosphoglyceric acid are decreased to glyceraldehyde-3-phosphates through the enzyme glyceraldehyde- 3-phosphate dehydrogenase along with the assistance of the light generated reducing power NADPH2.
Diagram: Calvin cycleThus, two molecules of NADPH2 will be consumed throughout this reaction. To decrease one molecule of CO2 equal to reduction two ATP and two NADPH2 are consumed.
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