Protein-Lecithin as carrier:
Bennet-Clark mentioned that the carrier can be protein related with a phosphatide example: Lecithin that carries both cations and anions and forms a lecithin-ion complex. This shifts to the internal side of membrane, discharges the ion by hydrolysis forming phosphatidic acid and choline due to the action of the enzyme lecithinase. Lecithin is renewed with the aid of enzymes Choline esterase and choline acetylase. This needs expenditure of the metabolic energy.
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If we intimately monitor these limitations it can be understood that these are the operational problems and not essential problems in the system.
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