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  Crossing Techniques

Crossing Techniques:

As garden pea is self pollinating, huge care was taken to see that:

  • The parents were weakened to prevent self pollination.
  • The anthers were composed from male parent and dusted onto the female parent and the stigma was bagged.
  • The seeds were collected individually in marked bottles.
  • Reciprocal crosses, (by interchanging the female and male parents) were conducted to confirm that there was no change in the ratio of the offsprings that is, sex has no influence on inheritance.

The plants employed as parents symbolize the parental generation designated as P. The resultant progeny from crossing of parents is termed first filial generation designated as F1 and progeny resultant from selfing the F1 plants was termed as second filial generation, symbolized as F2.

Crosses including inheritance of only one pair of contrasting characters are termed as monohybrid crosses and those including two pairs of contrasting characters are termed as dihybrid crosses.

1847_crossing techniques.jpg

Figure: Contrasting characters of traits selected by Mendel

 

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