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  Precipitation

Precipitation:

Precipitation is the arrangement of soluble antigen along with specific antibody that leads to the creation of an insoluble aggregation. Immune precipitation takes place while antigen and antibody combine in solution and make a visible aggregate. Precipitation reaction is quantifiable. The difference in the ratio of antibody- antigen leads to dissimilar levels of lattice formation, and thus to different amounts of precipitate. This phenomenon, termed as the prozone phenomenon was antibody might excess, zone of equivalence of antigen- antibody/antigen may excess. Factors influence precipitations are temperature, pH, salt concentration and reaction volume.

Agglutination:

The clumping/agglutination of specific antigens through specific antibodies. Clumping results in the creation of a lattice where antigen and antibody are cross linked. Agglutination methods are semi qualitative or quantitative at best and its reaction can be employed in several applications as it poses a high degree of sensitivity. Agglutination reactions can be categorized as either direct or indirect.

In the indirect agglutination a molecule is normally soluble is attached to a particle and rendered insoluble.

In the direct agglutination reaction, the antigenic determinant is a usual component of the particle surface.

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                                                      Diagram: Process of agglutination

 

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