Significance of metabolic flux analysis


Answer the following questions.

Question 1) Describe NMR and IR spectroscopy have been proven to be valuable techniques in structural biology.

Question 2) With the help of schematic diagram, describe the components and working of spectro fluorometer.

Question 3) Describ with the help of appropriate example, how pathway manipulation leads to qualitative and/or quantitative change in the final product.

Question 4) Explain the role of HPLC and GLC in analysis of a metabolite.

Question 5) What do you mean by metabolic flux analysis? Mention, with appropriate example, its significance.

Question 6) Illustrate the principle of isopycnic density gradient centrifugation. Mention its applications.

Question 7) Enlist the types of terpenoids mention four major steps involved in the biosynthesis of terpenoids.

Question 8) Describe 2D gel electrophoresis as an appropriate tool to study proteins.

Question 9) Compare and contrast integral proteins and peripheral proteins with respect to isolation, properties and functions.

Question 10) State the phytochemical methods for analysis of natural products and describe the methods.

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Biology: Significance of metabolic flux analysis
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