Physical Nitrogen or Non-Biological Fixation:

This includes fixation of nitrogen by chemical procedure in industry or naturally by electrical discharges like lightning.

Biological Nitrogen Fixation:

Nitrogen fixation occurring by living things is termed as biological nitrogen fixation. These comprise the bacteria and blue-green algae that have acquired the capacity to fix atmospheric nitrogen during the evolutionary procedure by possessing a set of genes termed as `nif' (Nitrogen fixing) genes. They fix Nitrogen in the given reaction.
 
Such organisms might be free living that are otherwise termed as non-symbiotic nitrogen fixers and might form symbiotic associations whenever they are termed as symbiotic nitrogen fixers.

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