--%>

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Problem on decinormal strength Can

    Can someone please help me in getting through this problem. How many grams of dibasic acid (having mol. wt. 200) must be present in 100ml  of its aqueous solution to provide decinormal strength: (i) 1g  (ii)2g  (iii) 10g  (iv) 20g<

  • Q : 6. 20 gm of hydrogen is present in 5

    6. 20 gm of hydrogen is present in 5 litre vessel. The molar concentration of hydrogen is

  • Q : Which solution will have highest

    Which solution will have highest boiling point:(a) 1% solution of glucose in water  (b) 1% solution of sodium chloride in water  (c) 1% solution of zinc sulphate in water  (d) 1% solution of urea in waterAnswer: (b) Na

  • Q : Strength of the Hydrochloric acid

    Provide solution of this question. 1.0 gm of pure calcium carbonate was found to need 50 ml of dilute HCL for complete reaction. The strength of the HCL solution is specified by : (a) 4 N (b) 2 N (c) 0.4 N (d) 0.2 N

  • Q : Latent heat of vaporization Normal

    Normal butane (C4H10) is stored as a compressed liquid at 90°C and 1400 kPa. In order to use the butane in a low-pressure gas-phase process, it is throttled to 150 kPa and passed through a vaporizer. The butane emerges from the vaporizer as a

  • Q : What do you mean by the term tripod

    What do you mean by the term tripod? Also state its uses?

  • Q : Explain the preparation of phenols. The

    The methods used for the preparation of phenols are given below:    From aryl sulphonic acids

  • Q : What is Spectroscopy? This is a very

    This is a very important aspect of Physical Chemistry in which knowledge of the size, shape, rigidity and electronic structure of molecules deduced from the experimental methods treated here goes hand in hand with the theoretical approaches of chemical reactions. Spec

  • Q : Einsteins mass energy relation In

    In Einstein’s mass energy relation e = mc2 for what is c employed or why is light needed for the reactions. As the reactions are with the help of neutrons?

  • Q : Molar concentration of hydrogen 20 g of

    20 g of hydrogen is present in 5 litre of vessel. Determine he molar concentration of hydrogen: (a) 4  (b) 1 (c) 3 (d) 2 Choose the right answer from above.