--%>

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Question based on vapour pressure and

    Give me answer of this question. The vapour pressure of water at 20degreeC is 17.54 mm. When 20g of a non-ionic, substance is dissolved in 100g of water, the vapour pressure is lowered by 0.30 mm. What is the molecular weight of the substances: (a) 210.2 (b) 206.88

  • Q : Problem on melting of ice A) It has

    A) It has been suggested that the surface melting of ice plays a role in enabling speed skaters to achieve peak performance. Carry out the following calculation to test this hypothesis. Suppose that the width of the skate in contact with the ice has been reduced by sh

  • Q : Question based on lowering of vapour

    Choose the right answer from following. The relative lowering of vapour pressure produced by dissolving 71.5 g of a substance in 1000 g of water is 0.00713. The molecular weight of the substance will be:  (a) 18.0 (b) 342 (c) 60 (d) 180

  • Q : Can protein act as the buffer Can

    Can protein act as the buffer? Briefly comment on that statement.

  • Q : Mole fraction Give me answer of

    Give me answer of following question. The sum of the mole fraction of the components of a solution is : (a) 0 (b) 1 (c) 2 (d) 4.

  • Q : Problem on reversible and irreversible

    The second law states that  dS ≥ (dQ/T), where dS = dQ/T for a reversible process and dS > dQ/T for an irreversible process.   a. Show that since dW12 = -dW21 (dWreverse = -dWforward) for a r

  • Q : Value of molar solution Select the

    Select the right answer of the question. Molar solution contains: (a)1000g of solute (b)1000g of solvent (c)1 litre of solvent (d)1 litre of solution

  • Q : Explain Photoelectron Spectroscopy. The

    The energies of both the outer and inner orbitals of atoms and molecules can be determined by photoelectron spectroscopy.Energy changes of the outermost or highest energy electron of molecules were dealt with here in a different passion. The energies of ot

  • Q : What do you mean by the term tripod

    What do you mean by the term tripod? Also state its uses?

  • Q : Atmospheric pressure Give me answer of

    Give me answer of this question. The atmospheric pressure is sum of the: (a) Pressure of the biomolecules (b) Vapour pressure of atmospheric constituents (c) Vapour pressure of chemicals and vapour pressure of volatile (d) Pressure created on to atmospheric molecules