You should read through the supplemental article on


Given the following information for the isocitrate dehydrogenase reaction: [NAD+]/[NADH] = 9, [a-ketoglutarate] = 0.1 mM, [isocitrate] = 0.02 mM, assume standard conditions for CO2. a) Look up the E'° for the appropriate half-reactions in your text and calculate the value of G'° based on those values. Determination of the reduction potentials for this enzyme is covered in one of the supplemental readings. You should read through the supplemental article on Isocitrate Dehydrogenase and see the lengths to which one must go in order to determine accurate physical quantities.

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